HDAC inhibitor sodium butyrate prevents allergic rhinitis and alters lncRNA and mRNA expression profiles in the nasal mucosa of mice.
Our previous studies showed that intranasal administration of a histone deacetylase inhibitor sodium butyrate (NaB) demonstrated a therapeutic effect in a mouse model of allergic rhinitis (AR). However, whether the AR NaB effective when administered orally and prophylaxis, as well as the potential effects on gene expression, remains unknown. This study aims to determine the preventive effects of NaB in AR when added to the newly weaned mice a diet and to evaluate changes in the length of the non-coding (Inc.) RNA and mRNA expression profiles in the nasal mucosa.
Rats were randomly divided into three groups as follows: i) Control (C) group (no treatment); ii) AR group [treated with ovalbumin (OVA)]; and iii) NaB + AR group (treated with OVA and NaB). NaB + AR group was given NaB in their feed (30 g / kg chow), while the other two groups were fed a normal feed between 3 and 6 weeks of age. At 7 weeks of age, the administration of OVA starts to push the AR in AR group and NaB + AR. Following the formation of models, behavioral assessment, western blotting and gene expression analysis performed. NaB exhibited preventive effect in a murine model AR, reduced the increase in histone deacetylase 1 (HDAC1) and HDAC8 expression and an increase in OVA-induced acetylation of histone H3 at lysine 9.
In addition, the increase AR-related NaB low expression of interleukin 2 (IL-2) , interferon-γ and IL-17 and IL-lowering expression of 4 , IL-5 and transforming growth factor β1. Gen Ontology and pathway analysis revealed the top 10 lines between groups. Octamer – bind transcription factor 1, ecotropic viral integration site 1 and box paired 4 predicted to be a target gene lncRNA (NONMMUT057309). Thus, NaB may show a preventive effect on AR. Additionally, lncRNA and mRNA expression profiles in rat nasal mucosa with AR significantly different after NaB treatment. These results may provide insight into the pathogenesis of AR and suggest new treatment targets.
HDAC inhibitor sodium butyrate prevents allergic rhinitis and alters lncRNA and mRNA expression profiles in the nasal mucosa of mice.
Ethyl-p-methoxycinnamate Improve and strengthen pluripotency Oct4 expression through the NF-kB signaling pathway.
The stem cells are pluripotent which have therapeutic applications in regenerative medicine and drug discovery. However, stem cell differentiation in vitro and inhibit the production of large-scale clinical application. Maintenance of cell pluripotency depends upon a complex network of transcription factor ; This, octamer – bind transcription factor – 4 (October 4 ) plays a key role. This study aims to establish an October 4 promoter-driven reporter gene firefly luciferase and screen small molecule compounds could maintain cell self-renewal and pluripotency.
The results showed that ethyl-p-methoxycinnamate (EPMC) increase promoter activity in October 4 gene, upregulated in October 4 at both mRNA and protein levels, and significantly promoted the formation of colonies P19 cells.
Description: A sandwich ELISA for quantitative measurement of Human (IgM) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human (IgM) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The Rheumatoid Factor (RF) IgM ELISA test system is intended for the qualitative and semi-quantitative detection of RF IgM-class antibody in human serum. The test system is intended to be used as an aid in the diagnosis of rheumatoid arthritis.
Description: Rheumatoid Factor IgM is an indirect solid phase enzyme immunoassay ( ELISA ) for the quantitative measurement of IgM class rheumatoid factor antibodies in human serum or plasma. The assay is intended for in vitro diagnostic use only as an aid in the determination of rheumatoid arthritis ( RA ).
Description: The Human HDV IgM ELISA Kit is an enzyme-linked immunosorbent assay for qualitative determination of IgM-class antibodies to hepatitis D virus in human serum or plasma.
Description: A sandwich ELISA for quantitative measurement of Human SRBC IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human SRBC IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human SRBC IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Membrane IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Membrane IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Membrane IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Description of target: Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <= 3.0 ng/mL
Description: A sandwich ELISA for quantitative measurement of Human Lebtospira IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Lebtospira IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Lebtospira IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The Cardiolipin Antibody Test System is an enzyme-linked immunosorbent assay (ELISA) designed for the semiquantitative measurement of circulating IgM autoantibodies to cardiolipin.
Human anti cardiolipin antibody IgM,ACA IgM ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human anti cardiolipin antibody IgM,ACA IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human anti cardiolipin antibody IgM,ACA IgM ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human anti cardiolipin antibody IgM,ACA IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human anti cardiolipin antibody IgM,ACA IgM ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human anti cardiolipin antibody IgM,ACA IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human epidemic hemorrhagic fever IgM,EHF IgM ELISA Kit
Description: EV-71 IgM ELISA isan enzyme-linked immunosorbent assay for qualitative determination of IgM-class antibodies to human enterovirus-71 (EV71) in serum or plasma samples. The assay is intended to be used in clinical laboratories for early diagnosis and management of patients related to infection with EV71.
Description: A sandwich ELISA for quantitative measurement of Human anti antibody(IgM) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human anti antibody(IgM) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human anti antibody(IgM) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Toxoplasma Gondii IgM Antibody (T. Gondii IgM) ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Human Hsp60 antibody (IgM) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Hsp60 antibody(IgM) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Qualitativeindirect ELISA kit for measuring Human platelet antibodies IgM (PA-IgM) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Qualitativeindirect ELISA kit for measuring Human platelet antibodies IgM(PA-IgM) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
These findings indicate that EPMC may reinforce self-renewal capacity of P19 cells. Pluripotency markers October 4 , SRY-related high-mobility group box protein-2, and Nanog are expressed at higher levels in the colonies EPMC-induced. EPMC can promote the formation of teratomas and P19 cell differentiation potential in vivo. It also enhanced self-renewal and pluripotency of human umbilical cord mesenchymal stem cells and mouse embryonic stem cells.
