Adult stem cells age during long-term culture in vitro, and neural stem cells (NSC), which can renew themselves and differentiate into neurons and glial cells, also in the show reduces the differentiation potential after repeated passaging. However, the mechanistic details underlying this process remains unclear.

In this study, we found that the long-term in vitro culture of NSCs resulted inflammatory- aging-associated upregulation and endoplasmic reticulum (ER) stress-related genes, including the proinflammatory cytokines interleukin (IL) 1β and IL6, which senescence- enzyme-linked matrix metallopeptidase 13 (MMP13), and ER stress-responsive transcription factor activating transcription factor 4 (ATF 4 ) and C / EBP homologous protein (CHOP).

However, the induction of cyclic and while the four reprogramming factor (POU domain, class 5, transcription factor 1, also known as octamer < / em> – bind transcription factor 4 ; SRY [sex determination region Y] -box 2; Kruppel – such as factor 4 , and myelocytomatosis oncogene; collectively referred to as OSKM) can inhibit the aging of the NSC, as indicated by a decrease in the expression of genes related to inflammation and ER stress.

We use ROSA- 4 F NSC, which revealed OSKM only one allele, to minimize the potential for full reprogramming or formation of tumors during NSC rejuvenation. We hope that this novel method of rejuvenation will increase the potential of NSCs as a clinical approach to the treatment of neurological diseases. True hermaphroditism with dysgerminomas: A case report ntroduction:

Inhibition of neural stem cell aging through the transient induction of reprogramming factors
Inhibition of neural stem cell aging through the transient induction of reprogramming factors

True hermaphroditism is a rare disorder and is usually sporadic.

It is defined by the presence of both ovaries and testes tissue together as ovotestis.Patient concerns: In this study, we report a rare case of true hermaphroditism with dysgerminomas. A 4 9 year old woman developed a mass in the inguinal region for 30 years. Recently three months, patients find that the size of the mass in her left inguinal region increased significantly.


Diagnosis: After surgical resection, the results of immunohistochemistry mass of the left reveals dysgerminomas with positive expression of placental alkaline phosphatase and octamer – bind transcription factor 3 / 4 , and the mass of the right is cryptorchidism a. chromosome analysis revealed a karyotype 4 6, XY. The combined immunohistochemistry and karyotype analysis, the diagnosis of true hermaphroditism with dysgerminomas made.


Intervention: Radiotherapy combined with chemotherapy after resection of the tumor used to improve his prognosis. Hormone replacement therapy with conjugated estrogens and medroxyprogesterone acetate is used to maintain her female characteristics.
Results: Patients undergoing hormone replacement and has been well over 6 months.

iFluor™ 610 succinimidyl ester

1038 1 mg
EUR 219

iFluor™ A7 SE

1039 1 mg
EUR 219

iFluor™ 546 succinimidyl ester

1048 1 mg
EUR 219

iFluor™ 568 succinimidyl ester

1049 1 mg
EUR 219

iFluor™ 450 maleimide

1057 1 mg
EUR 219

iFluor™ 647 amine

1074 1 mg
EUR 219

iFluor™ 680 amine

1076 1 mg
EUR 219

iFluor™ 700 amine

1077 1 mg
EUR 219

iFluor™ 710 amine

1078 1 mg
EUR 219

iFluor™ 750 amine

1079 1 mg
EUR 219

iFluor™ 680 hydrazide

1086 1 mg
EUR 219

iFluor™ 700 hydrazide

1087 1 mg
EUR 219

iFluor™ 750 hydrazide

1088 1 mg
EUR 219

ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

11001 1 L
EUR 202

ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

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EUR 480

ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*

11004 5x10 mL
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Amplite™ Blue

11005 25 mg
EUR 176

Amplite™ IR

11009 1 mg
EUR 132

Amplite™ Red

11011 1000 Assays
EUR 132

ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

11012 100 ml
EUR 115

ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1105 2 Labelings
EUR 176

Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*

11050 1 g
EUR 115

iFluor™ 488 tyramide

11060 1 mg
EUR 219

Azido-Cy5 tyramide

11061 1 mg
EUR 306

Amplite™ Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*

11100 200 Tests
EUR 219

Amplite™ Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

11105 500 tests
EUR 219

ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

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EUR 176

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ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1123 2 Labelings
EUR 176

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EUR 50

Amplite™ Colorimetric Glucose Oxidase Assay Kit

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1130 2 Labelings
EUR 176

Amplite™ Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

11300 500 Tests
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Amplite™ Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

11301 200 Tests
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11302 200 Tests
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11303 200 Tests
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11304 200 Tests
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11305 200 Tests
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11306 200 Tests
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11307 200 Tests
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1140 5 mg
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11400 200 Tests
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11401 200 Tests
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11402 200 Tests
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mFluor™ Violet 510 acid

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EUR 306

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mFluor™ Blue 570 acid

1143 5 mg
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mFluor™ Green 620 acid

1144 5 mg
EUR 306

mFluor™ Yellow 630 acid

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mFluor™ Violet 500 SE

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EUR 219

mFluor™ Violet 450 SE

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Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*

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Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

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EUR 263

mFluor™ Violet 510 SE

1151 1 mg
EUR 219

mFluor™ Violet 540 SE

1152 1 mg
EUR 219

Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

11540 1000 Tests
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Amplite™ Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

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Amplite™ Luminometric Peroxidase (HRP) Assay Kit

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EUR 219

Amplite™ Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

11560 200 Tests
EUR 306

mFluor™ Blue 570 SE

1160 1 mg
EUR 306

FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2*

11600 5 mg
EUR 132

MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*

11610 25 mg
EUR 115

MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*

11612 10 g
EUR 898

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*

11614 25 mg
EUR 115

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*

11617 5 g
EUR 480

pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9*

11619 25 mg
EUR 115

CF-MUP, sodium salt *Superior alternative to MUP*

11628 10 mg
EUR 50

mFluor™ Green 620 SE

1165 1 mg
EUR 306

mFluor™ Yellow 630 SE

1170 1 mg
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11700 1 mg
EUR 219

mFluor™ Red 780 SE

1191 1 mg
EUR 306

Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*

11950 500 Tests
EUR 176

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*

11952 500 Tests
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Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

11953 500 Tests
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Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence*

11954 500 Tests
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ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

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12481 25 mg
EUR 219

Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]

12482 100 mg
EUR 480

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D-Luciferin, free acid *CAS#: 2591-17-5*

12501 25 mg
EUR 115

D-Luciferin, free acid *CAS#: 2591-17-5*

12502 100 mg
EUR 132

D-Luciferin, free acid *CAS#: 2591-17-5*

12503 1 g
EUR 528

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12505 25 mg
EUR 115

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12506 100 mg
EUR 132

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12507 1 g
EUR 263

D-Luciferin, sodium salt *CAS#: 103404-75-7*

12509 25 mg
EUR 115

D-Luciferin, sodium salt *CAS#: 103404-75-7*

12510 100 mg
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D-Luciferin, sodium salt *CAS#: 103404-75-7*

12511 1 g
EUR 528

D-Luciferin phosphate *CAS 145613-12-3*

12512 1 mg
EUR 219

D-Luciferin galactoside *CAS 131474-38-9*

12513 5 mg
EUR 219

D-Luciferin methyl ester *CAS 73918-26-0*

12514 5 mg
EUR 219

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12515 5 mg
EUR 219

D-Luciferin acetate

12516 5 mg
EUR 219

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12518 1 plate
EUR 176

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12519 10 plates
EUR 393

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12520 100 plates
EUR 2616

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12530 1 plate
EUR 219

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12531 10 Plates
EUR 702

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12532 100 plates
EUR 3486

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12535 1 plate
EUR 219

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12536 10 plates
EUR 876

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

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1255 2 Labelings
EUR 176

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12551 200 Tests
EUR 393

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12560 1 mg
EUR 219

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12561 5 mg
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1260 2 Labelings
EUR 176

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

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Amplite™ Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

12602 200 Tests
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Amplite™ Colorimetric Beta-Galactosidase Assay Kit

12604 200 Tests
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HIS Lite™ Cy3 Bis NTA-Ni Complex

12610 1 mg
EUR 393

HIS Lite™ Cy5 Bis NTA-Ni Complex

12613 1 mg
EUR 393

ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1265 2 Labelings
EUR 176

ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1290 2 Labelings
EUR 176

ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1292 2 Labelings
EUR 176

ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

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EUR 176

ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1299 2 Labelings
EUR 176

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1300 2 Labelings
EUR 876

Benzoyl-Leu-Gly-Arg-Aminoluciferin

13000 1 mg
EUR 480

BOC-Leu-Gly-Arg-Aminoluciferin

13001 1 mg
EUR 480

BOC-Val-Pro-Arg-Aminoluciferin

13002 1 mg
EUR 480

D-VLK-AMC [D-Val-Leu-Lys-AMC]

13201 5 mg
EUR 132

Ac-DEVD-AFC *CAS 201608-14-2*

13401 5 mg
EUR 132

Ac-DEVD-AMC *CAS 169332-61-0*

13402 5 mg
EUR 132

Ac-DEVD-CHO *CAS 169332-60-9*

13403 1 mg
EUR 132

Ac-DEVD-pNA *CAS 189950-66-1*

13405 5 mg
EUR 132

Ac-IETD-AFC *CAS 211990-57-7*

13410 5 mg
EUR 219

Ac-IETD-AMC

13411 5 mg
EUR 219

Ac-IETD-CHO *CAS 191338-86-0*

13412 5 mg
EUR 219

Z-IETD-pNA *CAS 219138-21-3*

13413 5 mg
EUR 219

D-Aminoluciferin

13415 10 mg
EUR 219

Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*

1342 2 Labelings
EUR 306

Z-DEVD-AMC

13421 5 mg
EUR 176

Z-IETD-AFC *CAS 219138-02-0*

13425 5 mg
EUR 158

Ac-LEHD-AMC *CAS 292633-16-0*

13426 5 mg
EUR 176

(Ac-LEHD)2-R110

13427 1 mg
EUR 132

(Ac-IETD)2-R110

13431 1 mg
EUR 132

Z-DEVD-ProRed™ 620

13433 1 mg
EUR 306

Z-IETD-ProRed™ 620

13434 1 mg
EUR 306

Z-LEHD-ProRed™ 620

13435 1 mg
EUR 306

Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8*

13445 10 mg
EUR 176

Gly-Pro-AMC *CAS 115035-46-6*

13450 5 mg
EUR 132

(Suc-LLVY)2R110

13451 1 mg
EUR 132

Suc-LLVY-Aminoluciferin

13452 1 mg
EUR 219

Suc-LLVY-AMC

13453 1 mg
EUR 132

(Ac-ANW)2R110

13455 1 mg
EUR 219

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

13456 100 tests
EUR 306

Pro-AMC

13457 5 mg
EUR 132

Ala-AMC

13458 5 mg
EUR 132

D-Ala-AMC *CAS 201847-52-1*

13459 5 mg
EUR 132

MeO-Succ-Arg-Pro-Tyr-AMC

13460 5 mg
EUR 132

BOC-Val-Pro-Arg-AMC

13461 5 mg
EUR 132

(BOC-VPR)2R110

13462 1 mg
EUR 132

(Ac-KQL)2R110

13465 1 mg
EUR 132

(Z-LLE)2R110

13466 1 mg
EUR 132

(Ac-PAL)2R110

13467 1 mg
EUR 132

(Ac-WLA)2R110

13468 1 mg
EUR 132

Buccutite™ Rapid APC-iFluor™ 700 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*

1347 2 Labelings
EUR 306

SRB-VAD-FMK [Sulforhodamine B-VAD-FMK]

13472 25 Tests
EUR 132

mFluor™ 450-VAD-FMK

13475 25 Tests
EUR 132

mFluor™ 510-VAD-FMK

13476 25 Tests
EUR 132

Z-Gly-Pro-AMC *CAS 68542-93-8*

13477 5 mg
EUR 132

Benzoyl-Arg-AMC

13478 5 mg
EUR 132

Ac-Pro-Ala-Leu-AMC

13479 5 mg
EUR 132

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1350 2 Labelings
EUR 306

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

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EUR 219


Conclusion: Expressions positive of placental alkaline phosphatase and octamer – bind transcription factor 3 / 4 can 2 marker dysgerminomas diagnosis. Surgery combined with radiotherapy and chemotherapy could improve the prognosis dysgerminomas. In addition, hormone replacement therapy with conjugated estrogens and medroxyprogesterone acetate greatly help to maintain female characteristics of patients with true hermaphroditism