Adult stem cells age during long-term culture in vitro, and neural stem cells (NSC), which can renew themselves and differentiate into neurons and glial cells, also in the show reduces the differentiation potential after repeated passaging. However, the mechanistic details underlying this process remains unclear.
In this study, we found that the long-term in vitro culture of NSCs resulted inflammatory- aging-associated upregulation and endoplasmic reticulum (ER) stress-related genes, including the proinflammatory cytokines interleukin (IL) 1β and IL6, which senescence- enzyme-linked matrix metallopeptidase 13 (MMP13), and ER stress-responsive transcription factor activating transcription factor 4 (ATF 4 ) and C / EBP homologous protein (CHOP).
However, the induction of cyclic and while the four reprogramming factor (POU domain, class 5, transcription factor 1, also known as octamer < / em> – bind transcription factor 4 ; SRY [sex determination region Y] -box 2; Kruppel – such as factor 4 , and myelocytomatosis oncogene; collectively referred to as OSKM) can inhibit the aging of the NSC, as indicated by a decrease in the expression of genes related to inflammation and ER stress.
We use ROSA- 4 F NSC, which revealed OSKM only one allele, to minimize the potential for full reprogramming or formation of tumors during NSC rejuvenation. We hope that this novel method of rejuvenation will increase the potential of NSCs as a clinical approach to the treatment of neurological diseases. True hermaphroditism with dysgerminomas: A case report ntroduction:
Inhibition of neural stem cell aging through the transient induction of reprogramming factors True hermaphroditism is a rare disorder and is usually sporadic.
It is defined by the presence of both ovaries and testes tissue together as ovotestis.Patient concerns: In this study, we report a rare case of true hermaphroditism with dysgerminomas. A 4 9 year old woman developed a mass in the inguinal region for 30 years. Recently three months, patients find that the size of the mass in her left inguinal region increased significantly.
Diagnosis: After surgical resection, the results of immunohistochemistry mass of the left reveals dysgerminomas with positive expression of placental alkaline phosphatase and octamer – bind transcription factor 3 / 4 , and the mass of the right is cryptorchidism a. chromosome analysis revealed a karyotype 4 6, XY. The combined immunohistochemistry and karyotype analysis, the diagnosis of true hermaphroditism with dysgerminomas made.
Intervention: Radiotherapy combined with chemotherapy after resection of the tumor used to improve his prognosis. Hormone replacement therapy with conjugated estrogens and medroxyprogesterone acetate is used to maintain her female characteristics.
iFluor™ 610 succinimidyl ester
1038
AAT Bioquest
1 mg
EUR 219
iFluor™ 546 succinimidyl ester
1048
AAT Bioquest
1 mg
EUR 219
iFluor™ 568 succinimidyl ester
1049
AAT Bioquest
1 mg
EUR 219
ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11001
AAT Bioquest
1 L
EUR 202
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11003
AAT Bioquest
1 L
EUR 480
ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
11004
AAT Bioquest
5x10 mL
EUR 132
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
11012
AAT Bioquest
100 ml
EUR 115
ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1105
AAT Bioquest
2 Labelings
EUR 176
Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*
11050
AAT Bioquest
1 g
EUR 115
Amplite™ Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
11100
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
11105
AAT Bioquest
500 tests
EUR 219
ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1114
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1120
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1123
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1126
AAT Bioquest
2 Labelings
EUR 50
Amplite™ Colorimetric Glucose Oxidase Assay Kit
11299
AAT Bioquest
500 Tests
EUR 306
ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1130
AAT Bioquest
2 Labelings
EUR 176
Amplite™ Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
11300
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*
11301
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*
11302
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
11303
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*
11304
AAT Bioquest
200 Tests
EUR 219
Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit
11305
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Catalase Assay Kit *Red Fluorescence*
11306
AAT Bioquest
200 Tests
EUR 219
Amplite™ Colorimetric Xanthine Oxidase Assay Kit
11307
AAT Bioquest
200 Tests
EUR 219
Amplite™ Colorimetric Acetylcholinesterase Assay Kit
11400
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*
11401
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*
11402
AAT Bioquest
200 Tests
EUR 219
Amplite™ Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
11403
AAT Bioquest
200 Tests
EUR 219
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*
11503
AAT Bioquest
200 Tests
EUR 263
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*
11504
AAT Bioquest
100 Tests
EUR 263
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*
11505
AAT Bioquest
100 Tests
EUR 263
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
11506
AAT Bioquest
100 Tests
EUR 263
Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
11540
AAT Bioquest
1000 Tests
EUR 219
Amplite™ Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
11541
AAT Bioquest
1000 Tests
EUR 219
Amplite™ Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
11551
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
11552
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence*
11553
AAT Bioquest
500 Tests
EUR 306
Amplite™ Luminometric Peroxidase (HRP) Assay Kit
11559
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
11560
AAT Bioquest
200 Tests
EUR 306
FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2*
11600
AAT Bioquest
5 mg
EUR 132
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*
11610
AAT Bioquest
25 mg
EUR 115
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*
11612
AAT Bioquest
10 g
EUR 898
MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*
11614
AAT Bioquest
25 mg
EUR 115
MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*
11617
AAT Bioquest
5 g
EUR 480
pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9*
11619
AAT Bioquest
25 mg
EUR 115
CF-MUP, sodium salt *Superior alternative to MUP*
11628
AAT Bioquest
10 mg
EUR 50
Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
11950
AAT Bioquest
500 Tests
EUR 176
Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*
11952
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*
11953
AAT Bioquest
500 Tests
EUR 219
Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence*
11954
AAT Bioquest
500 Tests
EUR 219
Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
11956
AAT Bioquest
100 Tests
EUR 219
ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1227
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1235
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid iFluor™ 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1240
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid iFluor™ 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1245
AAT Bioquest
2 Labelings
EUR 176
Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]
12481
AAT Bioquest
25 mg
EUR 219
Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]
12482
AAT Bioquest
100 mg
EUR 480
ReadiLink™ Rapid iFluor™ 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1250
AAT Bioquest
2 Labelings
EUR 176
D-Luciferin, free acid *CAS#: 2591-17-5*
12501
AAT Bioquest
25 mg
EUR 115
D-Luciferin, free acid *CAS#: 2591-17-5*
12502
AAT Bioquest
100 mg
EUR 132
D-Luciferin, free acid *CAS#: 2591-17-5*
12503
AAT Bioquest
1 g
EUR 528
D-Luciferin, potassium salt *CAS#: 115144-35-9*
12505
AAT Bioquest
25 mg
EUR 115
D-Luciferin, potassium salt *CAS#: 115144-35-9*
12506
AAT Bioquest
100 mg
EUR 132
D-Luciferin, potassium salt *CAS#: 115144-35-9*
12507
AAT Bioquest
1 g
EUR 263
D-Luciferin, sodium salt *CAS#: 103404-75-7*
12509
AAT Bioquest
25 mg
EUR 115
D-Luciferin, sodium salt *CAS#: 103404-75-7*
12510
AAT Bioquest
100 mg
EUR 132
D-Luciferin, sodium salt *CAS#: 103404-75-7*
12511
AAT Bioquest
1 g
EUR 528
D-Luciferin phosphate *CAS 145613-12-3*
12512
AAT Bioquest
1 mg
EUR 219
D-Luciferin galactoside *CAS 131474-38-9*
12513
AAT Bioquest
5 mg
EUR 219
D-Luciferin methyl ester *CAS 73918-26-0*
12514
AAT Bioquest
5 mg
EUR 219
D-Luciferin ethyl ester *CAS 135251-85-3*
12515
AAT Bioquest
5 mg
EUR 219
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12518
AAT Bioquest
1 plate
EUR 176
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12519
AAT Bioquest
10 plates
EUR 393
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*
12520
AAT Bioquest
100 plates
EUR 2616
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12530
AAT Bioquest
1 plate
EUR 219
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12531
AAT Bioquest
10 Plates
EUR 702
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12532
AAT Bioquest
100 plates
EUR 3486
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12535
AAT Bioquest
1 plate
EUR 219
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12536
AAT Bioquest
10 plates
EUR 876
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12537
AAT Bioquest
100 plates
EUR 3486
ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1255
AAT Bioquest
2 Labelings
EUR 176
Amplite™ Colorimetric Beta-Lactamase Activity Assay Kit
12551
AAT Bioquest
200 Tests
EUR 393
BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]
12560
AAT Bioquest
1 mg
EUR 219
BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]
12561
AAT Bioquest
5 mg
EUR 393
ReadiLink™ Rapid iFluor™ 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1260
AAT Bioquest
2 Labelings
EUR 176
Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
12601
AAT Bioquest
500 Tests
EUR 306
Amplite™ Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
12602
AAT Bioquest
200 Tests
EUR 306
Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*
12603
AAT Bioquest
200 Tests
EUR 306
Amplite™ Colorimetric Beta-Galactosidase Assay Kit
12604
AAT Bioquest
200 Tests
EUR 306
HIS Lite™ Cy3 Bis NTA-Ni Complex
12610
AAT Bioquest
1 mg
EUR 393
HIS Lite™ Cy5 Bis NTA-Ni Complex
12613
AAT Bioquest
1 mg
EUR 393
ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1265
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1290
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1292
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1294
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1299
AAT Bioquest
2 Labelings
EUR 176
ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*
1300
AAT Bioquest
2 Labelings
EUR 876
Benzoyl-Leu-Gly-Arg-Aminoluciferin
13000
AAT Bioquest
1 mg
EUR 480
BOC-Leu-Gly-Arg-Aminoluciferin
13001
AAT Bioquest
1 mg
EUR 480
BOC-Val-Pro-Arg-Aminoluciferin
13002
AAT Bioquest
1 mg
EUR 480
D-VLK-AMC [D-Val-Leu-Lys-AMC]
13201
AAT Bioquest
5 mg
EUR 132
Ac-DEVD-AFC *CAS 201608-14-2*
13401
AAT Bioquest
5 mg
EUR 132
Ac-DEVD-AMC *CAS 169332-61-0*
13402
AAT Bioquest
5 mg
EUR 132
Ac-DEVD-CHO *CAS 169332-60-9*
13403
AAT Bioquest
1 mg
EUR 132
Ac-DEVD-pNA *CAS 189950-66-1*
13405
AAT Bioquest
5 mg
EUR 132
Ac-IETD-AFC *CAS 211990-57-7*
13410
AAT Bioquest
5 mg
EUR 219
Ac-IETD-CHO *CAS 191338-86-0*
13412
AAT Bioquest
5 mg
EUR 219
Z-IETD-pNA *CAS 219138-21-3*
13413
AAT Bioquest
5 mg
EUR 219
Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*
1342
AAT Bioquest
2 Labelings
EUR 306
Z-IETD-AFC *CAS 219138-02-0*
13425
AAT Bioquest
5 mg
EUR 158
Ac-LEHD-AMC *CAS 292633-16-0*
13426
AAT Bioquest
5 mg
EUR 176
Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8*
13445
AAT Bioquest
10 mg
EUR 176
Gly-Pro-AMC *CAS 115035-46-6*
13450
AAT Bioquest
5 mg
EUR 132
Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
13456
AAT Bioquest
100 tests
EUR 306
Buccutite™ Rapid APC-iFluor™ 700 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*
1347
AAT Bioquest
2 Labelings
EUR 306
SRB-VAD-FMK [Sulforhodamine B-VAD-FMK]
13472
AAT Bioquest
25 Tests
EUR 132
Z-Gly-Pro-AMC *CAS 68542-93-8*
13477
AAT Bioquest
5 mg
EUR 132
Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*
1350
AAT Bioquest
2 Labelings
EUR 306
Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*
13500
AAT Bioquest
500 Tests
EUR 219
Conclusion: Expressions positive of placental alkaline phosphatase and octamer – bind transcription factor 3 / 4 can 2 marker dysgerminomas diagnosis. Surgery combined with radiotherapy and chemotherapy could improve the prognosis dysgerminomas. In addition, hormone replacement therapy with conjugated estrogens and medroxyprogesterone acetate greatly help to maintain female characteristics of patients with true hermaphroditism
