Recent technological advancements in stem cell research for targeted therapeutics.
by Alyssa
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Stem cells have characteristics of self-renewal, pluripotency and differentiation, which is responsible for the addition of tissue or organ. Stem cells as a potential therapeutic tool in drug targeting and regenerative medicine of curing various neurological diseases and congenital diseases malignancy. These technological advances have formed a stem cell as the future of medicine.
However, because of ethico-social constraints, the use of embryonic stem cells (ESCs) have been avoided, while the physiological availability of adult stem cells (ASCs) and induced pluripotent stem cells (iPSCs) has obtained the right preferences. iPSCs are very similar to ESCs in terms of their self-renewal and pluripotency.
Here, we have summarized the technologies that have shaped the isolation of stem cells, molecular markers and factor is responsible for their maintenance. Different mobile ( transcription factor , protein regulation, miRNA as miRNA-296, miRNA-1 4 5, etc.) and components of the extracellular transcend stem fate cell. identification and characterization of them involves the development and efficient use of tools such as magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS). Several technologies have been patented and spin-off based on them have been commercialized.
In conclusion, we present the scope of the future and the possibility that stem cell technology look for us. Pictorial graphical abstract representation of a therapeutic approach to the treatment of diseases using stem cell technology. Disease-specific adult stem cells isolated with cell niche by utilizing tools such as FACS / MACS / LCM, etc. After that, the cells are programmed through the introduction Yamanaka factor (Oct3 / 4 , Sox2, c-myc, Klf 4 ) to create ips cells (iPSCs). The iPSCs certain diseases suffered genetic modifications after the delivery of therapeutic genes through retroviral vehicle.
The cells genetically modified with the disease was reintroduced to the therapeutic effects. FACS, fluorescence activated cell sorting; MACS, magnetic-activated cell sorting; LCM, laser capture microdissection; Oct3 / 4 , octamer – bind transcription factor 3 / 4 ; Sox2, sex determining region Y-box 2; Klf 4 , Kruppel like factor 4 .
Recent technological advancements in stem cell research for targeted therapeutics.
Oct4 expression in gastric carcinoma: correlation with tumor proliferation, angiogenesis and survival.
Octamer – bind transcription factor 4 (October 4 ) is transcription factor which has an important role in stem cell differentiation and self-renewal. October 4 has also been implicated in the tumorigenicity of different cancers. This study aimed to analyze the October 4 expression in gastric carcinoma (GC) and to evaluate the relationship between October 4 of expression and parameters of clinicopathological, tumor proliferation and angiogenesis in addition to patient survival.
October 4 mRNA was detected by reverse quantitative transcription PCR (qRT-PCR) in 4 specimen 5 GC and adjacent non tissues.We-cancer found a significant difference in October 4 the relative mRNA expression levels in GC tissues compared to non-cancerous tissue adjacent (p <0.001).
Description: A polyclonal antibody against LOXL1. Recognizes LOXL1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:1000-1:2000, WB:1:200-1:1000
Description: A polyclonal antibody against LOXL1. Recognizes LOXL1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Description: A polyclonal antibody against Loxl1. Recognizes Loxl1 from Mouse. This antibody is Unconjugated. Tested in the following application: ELISA
Description: This gene encodes a member of the lysyl oxidase family of proteins. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagen and elastin. The encoded preproprotein is proteolytically processed to generate the mature enzyme. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Mutations in this gene are associated with exfoliation syndrome.
Description: This gene encodes a member of the lysyl oxidase family of proteins. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagen and elastin. The encoded preproprotein is proteolytically processed to generate the mature enzyme. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Mutations in this gene are associated with exfoliation syndrome.
Description: This gene encodes a member of the lysyl oxidase family of proteins. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagen and elastin. The encoded preproprotein is proteolytically processed to generate the mature enzyme. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Mutations in this gene are associated with exfoliation syndrome.
Description: A polyclonal antibody against LOXL1. Recognizes LOXL1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Loxl1. Recognizes Loxl1 from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against LOXL1. Recognizes LOXL1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Loxl1. Recognizes Loxl1 from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LOXL1 - middle region. This antibody is tested and proven to work in the following applications:
Furthermore, immunohistochemistry (IHC) was performed to study the October 4 expression in the case of GC. High-October 4 immunostaining was detected in 62.2% of specimens GC.
