Induced pluripotent stem (iPS) cells are laboratory-produced cells that combine the advantages of biological and adult somatic stem cells for cell-based therapies. The reprogramming of cells, such as fibroblasts, into embryonic stem cell-like state is done by ectopic expression of transcription factor is responsible for generating embryonic stem cell properties.

This primer factor is octamer – bind transcription factor 4 (Oct3 / 4 ), sex determining region Y-box 2 (Sox2), Krüppel like factor 4 (Klf 4 ), and the proto-oncogene homolog protein from bird myelocytomatosis (c-Myc). Somatic cells can be easily obtained from the patient to be subjected to cell therapy and reprogrammed to obtain necessary high plasticity of embryonic stem cells.

These cells have no ethical limitations were involved, as in the case of embryonic stem cells, and displays minimal risk of immunologic rejection after transplantation. Currently, several ongoing clinical trials, most of them in Phase I or II. However, some of the inherent risks, such as chromosomal instability, insertional tumor, and teratoma formation, must be overcome to achieve full clinical translation. However, clinical trials and extensive basic research studying the biology of these cells, a promising future for the human cell-based therapy using iPS cells seem to be more clear and close.

Induced Pluripotent Stem Cells: Hope in the Treatment of Diseases, including Muscular Dystrophies
Induced Pluripotent Stem Cells: Hope in the Treatment of Diseases, including Muscular Dystrophies

Dexamethasone inhibits stemness Improving maintenance and chemosensitivity of hepatocellular carcinoma stem cells by inducing deSUMOylation HIF-1α and Oct4

It has been controversial whether patients with hepatocellular carcinoma (HCC) should receive glucocorticoid therapy during chemotherapy. Recent studies have shown that glucocorticoids increase the sensitivity of tumor therapy for some chemotherapy drugs, but the specific mechanism remains unclear. In this study, dexamethasone (Dex) is used for stem cells to treat HCC. The results showed that Dex reduced maintenance stemness and self-renewal of cells HCC stem, promoted the epithelial-to-mesenchymal transition, migration inhibiting and angiogenesis and, more importantly, increase the sensitivity of cells to herpes simplex virus thymidine kinase / ganciclovir system of medicine in vitro and in vivo.

Further mechanistic analysis showed that Dex inhibits small ubiquitin-like modifier (SUMO) modification of some proteins in HCC stem cells, including hypoxia inducible factor (HIF) -1α, protein hypoxia tolerance is important, and octamer – bind transcription factor 4 (October 4 ), stemness protein essential maintenance. DeSUMOylation encourage HIF-1α and October 4 reduced their accumulation in the nucleus, thereby inhibiting tumor angiogenesis and maintenance of stemness.

This finding provides a new perspective to study the mechanisms underlying the effects of anti-hepatocarcinogenesis of Dex. Because of the side effects of glucocorticoids on low-dose anti-inflammatory effect, the right combination of glucocorticoids and chemotherapy drugs are expected to improve the survival of HCC patients and their prognosis.Androgen deprivation therapy eventually lead to the development castration- resistant prostate cancer (CRPC)

iFluor™ 610 succinimidyl ester

1038 1 mg
EUR 219

iFluor™ A7 SE

1039 1 mg
EUR 219

iFluor™ 546 succinimidyl ester

1048 1 mg
EUR 219

iFluor™ 568 succinimidyl ester

1049 1 mg
EUR 219

iFluor™ 450 maleimide

1057 1 mg
EUR 219

iFluor™ 647 amine

1074 1 mg
EUR 219

iFluor™ 680 amine

1076 1 mg
EUR 219

iFluor™ 700 amine

1077 1 mg
EUR 219

iFluor™ 710 amine

1078 1 mg
EUR 219

iFluor™ 750 amine

1079 1 mg
EUR 219

iFluor™ 680 hydrazide

1086 1 mg
EUR 219

iFluor™ 700 hydrazide

1087 1 mg
EUR 219

iFluor™ 750 hydrazide

1088 1 mg
EUR 219

ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

11001 1 L
EUR 202

ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

11003 1 L
EUR 480

ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*

11004 5x10 mL
EUR 132

Amplite™ Blue

11005 25 mg
EUR 176

Amplite™ IR

11009 1 mg
EUR 132

Amplite™ Red

11011 1000 Assays
EUR 132

ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

11012 100 ml
EUR 115

ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1105 2 Labelings
EUR 176

Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*

11050 1 g
EUR 115

iFluor™ 488 tyramide

11060 1 mg
EUR 219

Azido-Cy5 tyramide

11061 1 mg
EUR 306

Amplite™ Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*

11100 200 Tests
EUR 219

Amplite™ Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

11105 500 tests
EUR 219

ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1114 2 Labelings
EUR 176

ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1120 2 Labelings
EUR 176

ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1123 2 Labelings
EUR 176

ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1126 2 Labelings
EUR 50

Amplite™ Colorimetric Glucose Oxidase Assay Kit

11299 500 Tests
EUR 306

ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1130 2 Labelings
EUR 176

Amplite™ Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

11300 500 Tests
EUR 219

Amplite™ Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

11301 200 Tests
EUR 219

Amplite™ Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

11302 200 Tests
EUR 219

Amplite™ Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

11303 200 Tests
EUR 219

Amplite™ Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*

11304 200 Tests
EUR 219

Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit

11305 200 Tests
EUR 219

Amplite™ Fluorimetric Catalase Assay Kit *Red Fluorescence*

11306 200 Tests
EUR 219

Amplite™ Colorimetric Xanthine Oxidase Assay Kit

11307 200 Tests
EUR 219

mFluor™ Violet 450 acid

1140 5 mg
EUR 219

Amplite™ Colorimetric Acetylcholinesterase Assay Kit

11400 200 Tests
EUR 219

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

11401 200 Tests
EUR 219

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*

11402 200 Tests
EUR 219

Amplite™ Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*

11403 200 Tests
EUR 219

mFluor™ Violet 510 acid

1141 5 mg
EUR 306

mFluor™ Violet 540 acid

1142 5 mg
EUR 306

mFluor™ Blue 570 acid

1143 5 mg
EUR 306

mFluor™ Green 620 acid

1144 5 mg
EUR 306

mFluor™ Yellow 630 acid

1145 5 mg
EUR 50

mFluor™ Red 700 acid

1146 5 mg
EUR 306

mFluor™ Violet 500 SE

1149 1 mg
EUR 219

mFluor™ Violet 450 SE

1150 1 mg
EUR 219

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*

11503 200 Tests
EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*

11504 100 Tests
EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*

11505 100 Tests
EUR 263

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

11506 100 Tests
EUR 263

mFluor™ Violet 510 SE

1151 1 mg
EUR 219

mFluor™ Violet 540 SE

1152 1 mg
EUR 219

Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

11540 1000 Tests
EUR 219

Amplite™ Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

11541 1000 Tests
EUR 219

Amplite™ Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

11551 500 Tests
EUR 219

Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

11552 500 Tests
EUR 219

Amplite™ Fluorimetric Peroxidase (HRP) Assay Kit *Near Infrared Fluorescence*

11553 500 Tests
EUR 306

Amplite™ Luminometric Peroxidase (HRP) Assay Kit

11559 500 Tests
EUR 219

Amplite™ Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

11560 200 Tests
EUR 306

mFluor™ Blue 570 SE

1160 1 mg
EUR 306

FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2*

11600 5 mg
EUR 132

MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*

11610 25 mg
EUR 115

MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2*

11612 10 g
EUR 898

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*

11614 25 mg
EUR 115

MUP [4-Methylumbelliferyl phosphate, free acid] *CAS 3368-04-5*

11617 5 g
EUR 480

pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 4264-83-9*

11619 25 mg
EUR 115

CF-MUP, sodium salt *Superior alternative to MUP*

11628 10 mg
EUR 50

mFluor™ Green 620 SE

1165 1 mg
EUR 306

mFluor™ Yellow 630 SE

1170 1 mg
EUR 50

ADP-ribose-pNP

11700 1 mg
EUR 219

mFluor™ Red 780 SE

1191 1 mg
EUR 306

Amplite™ Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*

11950 500 Tests
EUR 176

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*

11952 500 Tests
EUR 219

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

11953 500 Tests
EUR 219

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence*

11954 500 Tests
EUR 219

Amplite™ Luminometric Alkaline Phosphatase Assay Kit *Luminescence*

11956 100 Tests
EUR 219

ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1227 2 Labelings
EUR 176

ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1235 2 Labelings
EUR 176

ReadiLink™ Rapid iFluor™ 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1240 2 Labelings
EUR 176

ReadiLink™ Rapid iFluor™ 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1245 2 Labelings
EUR 176

Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]

12481 25 mg
EUR 219

Cyluc1 [(4S)-2-(6,7-Dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylic acid]

12482 100 mg
EUR 480

ReadiLink™ Rapid iFluor™ 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1250 2 Labelings
EUR 176

D-Luciferin, free acid *CAS#: 2591-17-5*

12501 25 mg
EUR 115

D-Luciferin, free acid *CAS#: 2591-17-5*

12502 100 mg
EUR 132

D-Luciferin, free acid *CAS#: 2591-17-5*

12503 1 g
EUR 528

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12505 25 mg
EUR 115

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12506 100 mg
EUR 132

D-Luciferin, potassium salt *CAS#: 115144-35-9*

12507 1 g
EUR 263

D-Luciferin, sodium salt *CAS#: 103404-75-7*

12509 25 mg
EUR 115

D-Luciferin, sodium salt *CAS#: 103404-75-7*

12510 100 mg
EUR 132

D-Luciferin, sodium salt *CAS#: 103404-75-7*

12511 1 g
EUR 528

D-Luciferin phosphate *CAS 145613-12-3*

12512 1 mg
EUR 219

D-Luciferin galactoside *CAS 131474-38-9*

12513 5 mg
EUR 219

D-Luciferin methyl ester *CAS 73918-26-0*

12514 5 mg
EUR 219

D-Luciferin ethyl ester *CAS 135251-85-3*

12515 5 mg
EUR 219

D-Luciferin acetate

12516 5 mg
EUR 219

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12518 1 plate
EUR 176

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12519 10 plates
EUR 393

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12520 100 plates
EUR 2616

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12530 1 plate
EUR 219

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12531 10 Plates
EUR 702

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12532 100 plates
EUR 3486

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12535 1 plate
EUR 219

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12536 10 plates
EUR 876

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12537 100 plates
EUR 3486

ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1255 2 Labelings
EUR 176

Amplite™ Colorimetric Beta-Lactamase Activity Assay Kit

12551 200 Tests
EUR 393

BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]

12560 1 mg
EUR 219

BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]

12561 5 mg
EUR 393

ReadiLink™ Rapid iFluor™ 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1260 2 Labelings
EUR 176

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

12601 500 Tests
EUR 306

Amplite™ Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

12602 200 Tests
EUR 306

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*

12603 200 Tests
EUR 306

Amplite™ Colorimetric Beta-Galactosidase Assay Kit

12604 200 Tests
EUR 306

HIS Lite™ Cy3 Bis NTA-Ni Complex

12610 1 mg
EUR 393

HIS Lite™ Cy5 Bis NTA-Ni Complex

12613 1 mg
EUR 393

ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1265 2 Labelings
EUR 176

ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1290 2 Labelings
EUR 176

ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1292 2 Labelings
EUR 176

ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1294 2 Labelings
EUR 176

ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

1299 2 Labelings
EUR 176

ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*

1300 2 Labelings
EUR 876

Benzoyl-Leu-Gly-Arg-Aminoluciferin

13000 1 mg
EUR 480

BOC-Leu-Gly-Arg-Aminoluciferin

13001 1 mg
EUR 480

BOC-Val-Pro-Arg-Aminoluciferin

13002 1 mg
EUR 480

D-VLK-AMC [D-Val-Leu-Lys-AMC]

13201 5 mg
EUR 132

Ac-DEVD-AFC *CAS 201608-14-2*

13401 5 mg
EUR 132

Ac-DEVD-AMC *CAS 169332-61-0*

13402 5 mg
EUR 132

Ac-DEVD-CHO *CAS 169332-60-9*

13403 1 mg
EUR 132

Ac-DEVD-pNA *CAS 189950-66-1*

13405 5 mg
EUR 132

Ac-IETD-AFC *CAS 211990-57-7*

13410 5 mg
EUR 219

Ac-IETD-AMC

13411 5 mg
EUR 219

Ac-IETD-CHO *CAS 191338-86-0*

13412 5 mg
EUR 219

Z-IETD-pNA *CAS 219138-21-3*

13413 5 mg
EUR 219

D-Aminoluciferin

13415 10 mg
EUR 219

Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*

1342 2 Labelings
EUR 306

Z-DEVD-AMC

13421 5 mg
EUR 176

Z-IETD-AFC *CAS 219138-02-0*

13425 5 mg
EUR 158

Ac-LEHD-AMC *CAS 292633-16-0*

13426 5 mg
EUR 176

(Ac-LEHD)2-R110

13427 1 mg
EUR 132

(Ac-IETD)2-R110

13431 1 mg
EUR 132

Z-DEVD-ProRed™ 620

13433 1 mg
EUR 306

Z-IETD-ProRed™ 620

13434 1 mg
EUR 306

Z-LEHD-ProRed™ 620

13435 1 mg
EUR 306

Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8*

13445 10 mg
EUR 176

Gly-Pro-AMC *CAS 115035-46-6*

13450 5 mg
EUR 132

(Suc-LLVY)2R110

13451 1 mg
EUR 132

Suc-LLVY-Aminoluciferin

13452 1 mg
EUR 219

Here, we demonstrate for the first time that histone H3K 4 SETD1A methyltransferase is the main regulator of the proliferation of metastatic CRPC (mCRPC). SETD1A expression was significantly correlated with survival rate of patients with prostate cancer.