Objective: The cancer stem-like cells (CSC) CSC markers and the role derived from papillary thyroid carcinoma (PTC) in the pathogenesis is unclear. This study aims to determine the nature of CSC using a ball tumor cells passaged but without sorting PTC CSC.


Materials and Methods: To identify the properties of CSC derived from PTC, the expression of SRY-box transcription factor 2 (Sox2), octamer – bind transcription factor 4 (OCT 4 ), Nanog homeobox (NANOG), thyroglobulin (TG), thyroid-stimulating hormone receptor (TSHR), E-cadherin, YES-related protein 1 (YAP1), and signal transducer and activator transcription < / em> 3 (STAT3) was investigated in the field of serial tumor passaged without sorting CSC.


Results: Ball cultured tumor had cancer stemness; High expression of October 4 , Sox2, NANOG, and YAP1; Low expression of E-cadherin; and varied expression of TG, TSHR, and STAT3. PTC tumors ball transfected with small interfering RNA targeting YAP1 possess less of the ball CSC non-transfected tumors are doing.


Conclusions: Tumor sphere cells derived from PTC by passaging without sorting CSC has more stem cell-like properties, and less differentiation potential. Thus, a simple and cost-effective method can be used for enrichment PTC stemness to work in cell-based models, reducing the need for the use of animal models. Impact OCT4 and Its Related Signaling Pathways in Cancer Gastrointestinal: Focused on Targeted Therapies There are many pieces of evidence support the effects of cancer stem cells in the initiation and progression of cancer.

Papillary Thyroid Cancer Tumor Spheres Cultured by Passaging Without Sorting Exhibit Cancer Stemness
Papillary Thyroid Cancer Tumor Spheres Cultured by Passaging Without Sorting Exhibit Cancer Stemness

However, the related mechanisms involved in this phenomenon is much more complicated to understand. Stemness different functions factor in the cancer stem cells (CSC) and their complex associations at different levels of cancer have been reported. Therefore, it seems that the focus on a single master factor will be more helpful to solve the puzzle singling pathway in these cells. Octamer – bind transcription factor 4 (OCT 4 ) is also known as POU domain, class 5, transcription factor 1 (POU5F1), one of these pluripotency the factor , has an important role in both embryogenesis and tumorigenesis.

In this review, we collect information about the relationship of different markers to October 4 of expression in the three types of gastrointestinal cancers including esophageal, gastric and colorectal cancers. October 4 via different signaling pathways have an impact on different processes of gastrointestinal cancers such as proliferation, invasion, and metastasis. Based on the literature, Oct. 4 have a profound effect on the development of cancer at different stages, therefore we suggest it has potential implications in the choice of therapy.

Although the pharmacological action of cardiovascular Tanshinone IIA (TanIIA) have been extensively studied, research on the role in the regeneration of the heart is still sufficient. This study used H9c2 heart myoblast cell line to evaluate the possibility TanIIA role in cardiac regeneration. It was found that certain concentrations of TanIIA inhibited cell proliferation by suppressing the expression of proteins associated with cell cycle [cyclin dependent kinase (CDK) 4 , CDK6 and cyclin D1] and proliferation [c-Myc, octamer – bind transcription factor 4 (October 4 ) and growing multiply cell nuclear antigen (PCNA)] without inducing apoptosis.

FluoroQuest™ Anti-fading Kit II *Optimized for Plate Imaging*

20003 1 kit
EUR 132
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Hamster IgG-Biotin conjugate, isotype control (Syrian)

20003-1-B 100 test
EUR 164

Hamster IgG, purified (Armenian, Isotype control)

20003-1AH 1 mg
EUR 263

G. Pig IgG Fc-Biotin conjugate (isotype control, non-immune) purified

20004-3-B 0.1 mg
EUR 225

G. Pig IgG-Biotin conjugate (isotype control)

20004-B 100 ug
EUR 202

Rat IgG F(ab')2 fragment, purified (isotype control)

20005-1-FAB2 1 mg
EUR 202

Rat IgG (Fc)-Biotin Conjuagte (isotype control), purified

20005-1-FC-B 0.1 mg
EUR 225

Rat IgG1-Biotin conjugate (isotype control)

20005-11-B 100 ug
EUR 202

Rat IgG2a-APC conjugate (isotype control)

20005-12-APC 25 tests
EUR 202

Rat IgG2a-Biotin conjugate (isotype control)

20005-12-B 100 ug
EUR 202

Rat IgG2b-Biotin conjugate (isotype control)

20005-13-B 100 ug
EUR 202

Rat IgG2c-Biotin conjugate (isotype control)

20005-14-B 100 ug
EUR 225

Rat IgM-Biotin conjugate (isotype control)

20005-21-B 100 ug
EUR 225

Rat IgG-Agarose conjugate(aff matrix)

20005-AS-1 0.5 ml
EUR 164

Rat IgG-Biotin conjugate (isotype control) (Isotype control)

20005-B 100 ug
EUR 164

Sheep IgG-Biotin Conjugate (isotype control, non-immune), purified

20006-1-B 0.5 mg
EUR 202

Sheep IgM-Biotin Conjugate (non-immune) control, purified

20006-2-B 0.1 mg
EUR 225

Sheep IgG Fc-Biotin Conjugate (isotype control, non-immune), purified

20006-4-B 0.5 mg
EUR 202

Human IgG-Biotin conjugate (isotype control, non-immune), purified

20007-1-B 0.5 mg
EUR 225

Human IgG (>98%, non-immune, control, Liquid @ 10 mg/ml, azide free, bulk size)

20007-1-BL-1 10 ml Ask for price

Human IgG (>98%, non-immune, control, powder, azide free, bulk size)

20007-1-BP-1 1 g
EUR 651

Human IgG (>98%, non-immune, control, powder, azide free, bulk size)

20007-1-BP-10 10 g
EUR 4313

Human IgG Fab fragment-Biotin Conjugate, purified

20007-1-FAB-B 0.1 mg
EUR 202

Human IgG F(ab')2 fragment, purified

20007-1-FAB2 1 mg
EUR 202

Human IgG Fab2 fragment-Biotin Conjugate, purified

20007-1-FAB2-B 0.1 mg
EUR 202

Human IgG Fc-Biotin conjugate (isotype control, non-immune), purified

20007-1-Fc-B 0.1 mg
EUR 164

Human IgG (Fc) fragment, purified (>95%, low endotoxin)

20007-1-FC-LE 100 ul
EUR 225

Human IgG (Fc) fragment-Biotin purified (>95%, low endotoxin)

20007-1-FC-LE-B 25 ug
EUR 286

Human IgG (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-1 1 mg
EUR 164

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-BTN 100 ug
EUR 286

TruStrip RDT Human IgG Rapid test cards, 10/pk

20007-1-RDT 1 Pk
EUR 171

TruStrip RDT Human IgG Rapid test cards, 25/pk

20007-1-RDT-25 1 Pk
EUR 293

Human IgM, purified (native, isotype control, >96% pure, ELISA Grade)

20007-2 1 mg
EUR 164

Human IgM purified (isotype control, >96% pure, ELISA Grade)

20007-2-10 10 mg
EUR 895

Human IgM purified (isotype control, >96% pure, ELISA Grade)

20007-2-5 5 mg
EUR 529

Human IgM (myeloma, >96%, non-immune, control; bulk size)

20007-2-B-100 100 mg Ask for price

Human IgM (myeloma, >96%, non-immune, control; bulk size)

20007-2-B-1000 1000 mg Ask for price

Human IgM-Biotin conjugate (myeloma, isotype control)

20007-2-MB 100 ug
EUR 164

Human IgM (Rheumatoid Factor plasma, >40,000 IU/ml, purifed for ELISA)

20007-2-RF 100 ul
EUR 469

Human IgE (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)

20007-3-LE-50 100 ug
EUR 408

Human IgE-Biotin (myeloma, kappa), purified (isotype control, >95%, low endotoxin, azide free)

20007-3-LE-BTN 100 ul
EUR 408

Human IgM Fab mu fragment (myeloma)-Biotin conjugate (isotype control)

20007-4-B 100 ug
EUR 225

Human IgG-Fc fragment (97%, low endotoxin, azide free)

20007-F-LE-100 100 ug
EUR 164

Human IgG-Fc fragment-Biotin (97%, low endotoxin, azide free)

20007-F-LE-BTN 100 ug
EUR 286

Human IgG1-Biotin Conjugate (-ve control)

20007-G1-B 100 ug
EUR 164

Recombinant (HEK) Human IgG1 Fc (103Cys/Ser, 99-330aa, His-tag, >95%, low endotoxin)

20007-G1-FC 50 ug
EUR 286

Human IgG2-Biotin Conjugate

20007-G2-B 100 ug
EUR 164

Recombinant (HEK) Human IgG2-Fc (257Ser/Ala, 99-327aa, His-tag, >95%, low endotoxin)

20007-G2-FC 50 ug
EUR 286

Human IgG3-Biotin Conjugate

20007-G3-B 100 ug
EUR 164

Recombinant (HEK) Human IgG3-Fc (99-377aa, His-tag, >95%, low endotoxin)

20007-G3-FC 50 ug
EUR 286

Human IgG4-Biotin Conjugate

20007-G4-B 100 ug
EUR 164

Recombinant (HEK) Human IgG4-Fc (108Ser/Pro, 99-327aa, His-tag, >95%, low endotoxin)

20007-G4-FC 50 ug
EUR 286

Mouse IgG F(ab')2 fragment, purified

20008-1-Fab2 1 mg
EUR 202

Mouse IgG (>97%, ELISA/RIA/Diagnostic Grade; -ve mouse viruses & Protein A/G free)

20008-1-WA 1 mg
EUR 164

Mouse IgG-Agarose conjugate (aff matrix)

20008-AS-1 0.5 ml
EUR 164

Mouse IgG-Biotin conjugate (isotype control)

20008-B 50 Tests
EUR 164

Mouse IgG-Biotin conjugate (isotype control)

20008-BT-1 1 mg
EUR 164

Mouse IgG Fab-Biotin conjugate (isotype control)

20008-Fab-B 100 ug
EUR 164

Mouse IgG F(ab')2-Biotin conjugate (isotype control)

20008-Fab2-B 100 ug
EUR 164

Mouse IgG F(ab')2-FITC conjugate (isotype control)

20008-Fab2-F 100 ug
EUR 164

Mouse IgG F(ab')2-HRP conjugate (isotype control)

20008-Fab2-HP 100 ug
EUR 164

Mouse IgG F(c)-Biotin conjugate (isotype control)

20008-Fc-B 100 ug
EUR 164

Rabbit IgG Fab-Biotin Conjugate, purified

20009-1-FAB-B 0.1 mg
EUR 202

Rabbit IgG F(ab')2 fragment, purified

20009-1-FAB2 1 mg
EUR 202

Recombinant (HEK) Rabbit IgG (Fc) (101-323aa, 185Thr/Asn284Ser, ~33 kda, >95%, Low Endotoxin)

20009-1-R-FC 50 ug
EUR 286

Rabbit IgM-Biotin Conjugate (isotype control, non-immune), purified

20009-2-1-B 0.1 mg
EUR 225

Rabbit IgA-Biotin Conjugate (isotype control, non-immune), purified

20009-3-1-B 0.1 mg
EUR 408

Rabbit IgG-Agarose conjugate

20009-AS-1 0.5 ml
EUR 164

Rabbit IgG (Whole, non-immune)-Biotin conjugate

20009-B 100 ug
EUR 164

Rabbit IgG-biotinylated (isotype control)

20009-BT-1 1 mg
EUR 286

Rabbit IgG F(ab')2-biotin conjugate, isotype control

20009-FAb2-B 100 ug
EUR 164

Rabbit IgG F(ab')2-FITC conjugate, isotype control

20009-FAb2-F 100 ug
EUR 164

Rabbit IgG F(ab')2-HRP conjugate, isotype control

20009-FAb2-HP 100 ug
EUR 164

Rabbit IgG F(ab')2-R-PE conjugate, isotype control

20009-FAb2-PE 100 tests
EUR 225

Rabbit IgG F(c)-biotin conjugate, isotype control

20009-Fc-B 100 ug
EUR 164

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder

20009-WA-1 1 mg
EUR 141

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder

20009-WA-5 5 mg
EUR 286

Human Insulin Antibody

20010-05011 150 ug
EUR 217

Human Insulin Antibody (Biotin Conjugate)

20010-05021 150 ug
EUR 276

Chicken IgG-Biotin conjugate (isotype control, non-immune), purified

20010-1-B 0.5 mg
EUR 225

Chicken IgM-Biotin conjugate (isotype control, non-immune) purified

20010-2-1-b 0.1 mg
EUR 225

Chicken IgG-Fc-Biotin conjugate (isotype control, non-immune), purified

20010-3-B 0.1 mg
EUR 225

Human Synaptobrevin-2 Antibody

20011-05011 150 ug
EUR 217

Human Synaptobrevin-2 Antibody (Biotin Conjugate)

20011-05021 150 ug
EUR 276

Human Synaptobrevin-2 AssayLite Antibody (FITC Conjugate)

20011-05041 150 ug
EUR 428

Human Synaptobrevin-2 AssayLite Antibody (RPE Conjugate)

20011-05051 150 ug
EUR 428

Human Synaptobrevin-2 AssayLite Antibody (APC Conjugate)

20011-05061 150 ug
EUR 428

Human Synaptobrevin-2 AssayLite Antibody (PerCP Conjugate)

20011-05071 150 ug
EUR 471

Goat IgG FAB fragment-Biotin conjugate

20011-1-FAB-B 100 tests
EUR 225

Goat IgG F(ab')2 fragment, purified

20011-1-FAB2 1 mg
EUR 164

Goat IgG-Agarose conjugate(aff matrix)

20011-AS-1 0.5 ml
EUR 164

Goat IgG (Whole, non-immune)-Biotin conjugate

20011-B 100 ug
EUR 164

Goat IgG F(ab')2 fragment-Biotin conjugate

20011-FAB2-B 100 tests
EUR 202

Goat IgG F(ab')2 fragment-Cy5 conjugate

20011-FAB2-Cy5 50 tests
EUR 202

Goat IgG F(ab')2 fragment-FITC conjugate

20011-FAB2-F 100 tests
EUR 202

Goat IgG FAB(ab')2 fragment-HRP conjugate

20011-FAB2-HP 100 tests
EUR 202

Goat IgG F(ab')2 fragment-R-PE conjugate

20011-FAB2-PE 50 tests
EUR 202

Goat IgG (fc)-Biotin conjugate (isotype control)

20011-Fc-B 100 ug
EUR 164

In this process, the expression of cardiac troponin in the treated cells was significantly increased and the migration of cells to the wound area to be treated is significantly improved.